Part:BBa_K273015:Design

PLlac - RBS - pdc - RBS - adh2(Y) - Ter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1096
  Illegal AgeI site found at 1243
  Illegal AgeI site found at 1621
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 130
  Illegal SapI site found at 544
  Illegal SapI site found at 1207
  

Design Notes

Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae was used instead of the adh2 from Z. mobilis

Source

Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191 Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae Assembled by the Uppsala iGEM Team 2009